Stem Cell Conditioned Medium Therapy:
Personalized Treatments Based on Cell Source and Administration Method

Stem cell conditioned medium therapy is an advanced medical option in regenerative medicine and anti-aging care. By collecting active factors secreted by stem cells during culture, this treatment offers a new choice for full-body anti-aging, health maintenance, and rejuvenation. [1][5]

Stem cell conditioned media from different sources—such as adipose tissue, dental pulp, umbilical cord, bone marrow, placenta, and amnion—contain unique growth factor profiles. By matching these sources with the right delivery method, we can target specific areas to meet your personal health and beauty needs. [1]

At Tokyo Relife Clinic, we offer individual treatments for full-body anti-aging, skin rejuvenation, scalp care, and joint repair. We also provide specialized combination programs for female hormone balance and ovarian rejuvenation. Our experienced physicians will design a custom plan tailored to your needs, including the right cell source, dosage, and delivery methods.

What is Stem Cell Conditioned Medium?

Stem cell conditioned medium is a active liquid extracted and purified during the in vitro culturing of stem cells.

Although it contains no whole cells or blood components, it is rich in:

• Growth factors (such as VEGF, HGF, and bFGF): Help promote new blood vessel growth and tissue regeneration.
• Cytokines: Help regulate immunity and inflammation.
• Exosomes: Act as key messengers that transmit signals between cells.

These active factors work at the cellular level to help activate the natural repair and regeneration processes of damaged or aging cells. They help restore youthful vitality throughout the body safely and naturally.

Recommended For and Treatment Areas

Due to its anti-inflammatory properties, support for blood vessel growth, cell repair, and immune regulation, this therapy is widely used in advanced medicine and anti-aging:

• Full-Body Anti-Aging & Rejuvenation: Helps relieve deep chronic fatigue, increase energy levels, and balance immune and endocrine functions.
• Dermatology & Aesthetic Care: Helps hydrate the skin, improve dullness, smooth fine lines, restore skin elasticity, and repair damaged or sensitive skin barriers. [5]
• Scalp & Hair Care: Helps activate hair follicles, balance the hair growth cycle, and improve scalp blood circulation for healthier hair. [8]
• Female Reproductive Health & Ovarian Support: Helps condition the ovarian microenvironment and support AMH (ovarian reserve) levels. [3][4]
• Menopause Symptom Support: Helps balance the internal environment, reduce low-grade chronic inflammation, and balance the nervous system to ease symptoms such as hot flashes, night sweats, insomnia, and mood changes.
• Organ & Joint Function Support: Helps ease knee pain and discomfort, improve microcirculation, and support respiratory and nervous system recovery. [6]
• Male Sexual Health Support (ED Care): Helps support blood vessel function in the penile area, reduce local inflammation, and protect nerve tissue.
• Brain Function & Fatigue Care: Offers neuroprotective support to help ease brain fatigue and maintain mental clarity and focus.

Core Advantages of Therapy at Tokyo Relife Clinic

Recent research shows that stem cells from different tissues produce conditioned media with different types and concentrations of active ingredients (growth factors, cytokines, and exosomal microRNAs). Matching the right source to your personal needs is essential for the best results.

Types of Stem Cell Conditioned Media and Their Features

We offer high-quality sources including adipose tissue, dental pulp, umbilical cord, bone marrow, placenta, and amnion. [1] Each source offers unique benefits:

1. Adipose-Derived

• Key Features: Rich in VEGF, HGF, and bFGF to support new blood vessel growth and tissue repair. Excellent for skin barrier restoration. [5]
• Main Focus: Skin rejuvenation, overall anti-aging, reducing wrinkles and sagging, improving local circulation, and scalp care. [7]

2. Dental Pulp-Derived

• Key Features: Derived from ectodermal tissue and rich in neurotrophic factors like BDNF and NGF. Focuses on nerve cell repair and controlling micro-inflammation. [9]
• Main Focus: Brain function support, central and peripheral nervous system care, recovery support after brain injury or stroke, nerve repair, and relieving mental fatigue.

3. Umbilical Cord-Derived

• Key Features: Extracted from perinatal tissue. Cells are highly primitive and active, offering strong full-body anti-inflammatory and immune-balancing effects. [10]
• Main Focus: Deep full-body anti-aging, chronic fatigue support, immune balance, joint repair, and easing poor circulation or cold sensitivity.

4. Bone Marrow-Derived

• Key Features: One of the most classic sources in regenerative medicine, supported by extensive clinical research in bone and cartilage repair. [6]
• Main Focus: Joint pain and age-related joint discomfort, easing chronic inflammation, and creating an environment for bone and cartilage self-repair.

5. Placental-Derived (Focus: Ovarian Health and Quality)

• Key Features: Designed for female reproductive health. Helps reduce ovarian inflammation, protect granulosa cells, promote micro-blood vessel growth, and improve the environment for developing follicles. Supports AMH and E2 (estrogen) levels while helping lower FSH. [12]
• Main Focus: Low ovarian function, premature ovarian insufficiency (POI), menopausal support, hormone balance, and deep skin anti-aging.

6. Amniotic-Derived (Focus: Follicle Quantity and Reserve)

• Key Features: Offers strong cell protection to help prevent early depletion of the follicle pool, supporting normal follicle development and targeting ovarian reserve support (promoting AMH↑, E2↑, and lower FSH↓). [13]
• Main Focus: Low ovarian reserve (low AMH), reduced follicle count, and age-related or inflammatory tissue support.

Dual Combination Therapy Examples

1. Amniotic × Bone Marrow (Anti-inflammatory & Tissue Repair)

• Synergistic Effect: Combines the immune-regulating properties of amnion with the tissue repair features of bone marrow to help ease local and systemic chronic inflammation and support skin and tissue recovery.
• Recommended For:
  ・Those with damaged skin barriers (such as severe acne marks or skin sensitivity) looking for deep repair. [6][7]
  ・Those looking to support their immune balance and health.

2. Amniotic × Placental (Skin Rejuvenation & Full-Body Anti-Aging)

• Synergistic Effect: Supports ovarian function and hormone balance while promoting blood vessel growth and cell renewal to improve skin elasticity, tone, and full-body vitality. [2]
• Recommended For:
  ・Those experiencing menopausal symptoms or severe hormone imbalance.
  ・Those seeking to relieve chronic fatigue and achieve full-body anti-aging.
  ・Those wanting clearer, more radiant, and youthful skin.

3. Bone Marrow × Placental (Full-Body Systemic Support)

• Synergistic Effect: Provides comprehensive care for bones, cartilage, blood vessels, circulation, and the endocrine system to address age-related decline. [6]
• Recommended For:
  ・Those concerned about age-related loss of physical vitality and body function.
  ・Those wanting to address multiple signs of aging like low energy, joint discomfort, and hormone imbalance at once.

4. Umbilical Cord + Placental (Special Focus: Complete Ovarian Support)

• Synergistic Effect: Combines two strong sources to boost blood vessel formation and tissue repair, helping ease local ovarian inflammation and balance immunity for overall anti-aging. [3]
• Recommended For:
  ・Those with significantly low ovarian function, early menopause, or signs of premature aging.
  ・Those with severe menopausal symptoms or hormone imbalances.
  ・Those looking for advanced anti-aging care to improve skin firmness.

5. Umbilical Cord + Amniotic (Special Focus: Ovarian Reserve Improvement)

• Synergistic Effect: Maximizes cell renewal and offers strong anti-inflammatory protection to help reduce tissue damage and revitalize full-body and reproductive health.
• Recommended For:
  ・Those with low ovarian reserve (low AMH) looking to support follicle quality and reserve. [4]
  ・Those with cold hands and feet due to poor circulation, or severe chronic fatigue.
  ・Those concerned about tissue damage caused by age or chronic inflammation.

Administration Methods and Treatment Goals

1. Intravenous (IV) Drip

• Available Sources: Adipose, Dental Pulp, Umbilical Cord
• Features & Goals: Delivers active factors throughout the body via the bloodstream. Ideal for overall body conditioning, easing deep fatigue, and supporting full-body anti-aging.

2. Targeted Local Injection (Joints / Specific Areas / Male Health)

• Available Sources: Adipose, Umbilical Cord, Bone Marrow, Amniotic, Placental (Determined after doctor evaluation)
• Features & Goals: Delivers high concentrations directly to target areas.
  ・Joints & Body Areas: Focuses on tissue repair and easing inflammation for age-related or exercise-related joint pain and mobility issues.
  ・Male Health (ED Care): Injected into the local area to help improve blood flow, vascular function, and nerve recovery.

3. Mesotherapy & Micro-Needling Injections

• Available Sources: Adipose, Umbilical Cord, High-Activity Composite Medium (Determined after doctor evaluation)
• Features & Goals:
  ・Face & Neck: Injected into the dermis layer to improve skin elasticity, radiance, and smoothness, helping fade fine lines. We also offer a combination treatment with Botox.
  ・Scalp Care: Applied directly to the scalp to nourish hair follicles and improve the scalp environment. Ideal for thinning hair, fine hair, or scalp anti-aging.

4. Medical-Grade Inhalation Therapy

• Available Sources: Umbilical Cord (Can be combined with Dental Pulp)
• Features & Goals: Uses a medical nebulizer to convert the solution into fine droplets for direct inhalation into the respiratory system. Ideal for lung and respiratory wellness. Home care options are also available under a doctor’s guidance.

5. Targeted Nasal Drops

• Available Sources: TRC Original High-Purity Adipose-Derived
• Features & Goals: Administered through the nasal mucosa to reach the central nervous system and brain directly via the nose-to-brain pathway. Home care options are available to help relieve brain fatigue, support nerve health, and improve sleep quality.

Clinical Research Reference Data

Stem cell conditioned medium is a liquid produced during the culture of stem cells and contains exosomes, various growth factors, and cytokines. Although it is attracting interest in regenerative medicine, research in humans is still limited, and further verification is ongoing.

*Note: The above are results from small-scale or exploratory studies. The ingredients, preparation methods, dosages, and administration methods differ between studies, and some studies involved combination with other treatments. These results do not guarantee the effects of treatment at our clinic.

Consultation and Treatment Steps

1. Medical Questionnaire (Approx. 10 min): Review your overall health, medical history, allergies, and treatment goals.
2. Doctor Consultation & Custom Plan (Approx. 20 min): A doctor evaluates your suitability, explains the options and delivery methods, and reviews the consent form with you.
3. Payment (Approx. 5 min): Cash, major credit cards, and mobile payment options are accepted.
4. Treatment Execution:
   ・IV Drip: Approx. 30 minutes
   ・Inhalation Therapy: Approx. 10 minutes
   ・Mesotherapy Injection (with numbing cream): Face + Neck (Approx. 75 min) / Face only (Approx. 60 min)
5. Post-Treatment Rest: Rest in the clinic for about 10 minutes after treatment. You may leave once the medical staff confirms everything is normal.

Precautions and Contraindications

This treatment is not available for individuals who:

• Are under 18 years of age.
• Are pregnant, breastfeeding, or planning to become pregnant soon.
• Have active malignant tumors or a recent history of cancer. *1
• Have active infectious diseases.
• Have severe allergies to specific antibiotics (penicillins, aminoglycosides, or macrolides).
• Have received a vaccine within the past month or plan to get vaccinated within the next 2 weeks.
• Are evaluated by a physician as unsuitable for this treatment.

*1 Patients currently undergoing cancer treatment or those still in follow-up observation after recovery cannot receive this treatment at this time.

Important Pre-Treatment Information

• Blood Donation Restrictions: In accordance with regulations by the Japanese Ministry of Health, Labour and Welfare, stem cell conditioned media are human-derived biological products. Due to the limitations of current testing methods, the risk of unknown viruses cannot be 100% ruled out. Therefore, anyone who receives this treatment (including IV drips) will no longer be eligible to donate blood or organs in Japan.
• Safety and Quality Standards: All stem cell conditioned media used at our clinic pass strict quality testing. They test negative for general bacteria, fungi, mycoplasma, Hepatitis B (HBV), Hepatitis C (HCV), HIV, HTLV, Parvovirus B19, EB virus, Cytomegalovirus, Herpes simplex, Varicella-zoster, Adenovirus, HPV, and bovine serum albumin. Strict screening for pathogens is performed to ensure high treatment safety.

Potential Side Effects and Recovery

• A small number of sensitive individuals may experience mild allergic reactions, such as temporary redness or itching.
• Injection sites may show slight pain, minor bruising, or mild swelling, which usually subsides naturally within a few days.
• Individual reactions may vary. Please make sure you fully understand these potential risks before treatment.
• If you feel any discomfort during or after treatment, please inform our staff immediately for proper evaluation and care.

If you are interested in stem cell conditioned medium therapy or our specialized female hormone and ovarian rejuvenation programs, please contact Tokyo Relife Clinic for a private consultation.